About training



Bioinformatics uses tools from computer science, mathematics, statistics, and engineering to answer questions about biological systems on a large scale. It typically explores information-storing macromolecules such as DNA, RNA, and proteins. It can be used to explore evolutionary relationships, examine the genomes of organisms, learn about human, animal and plant diseases, and look at trends in populations. Now-a-days bioinformatics has become an integral part of biological research. Hence, it is necessary for the researchers in biological science to know the fundamentals of bioinformatics, its application in stimulating and modelling the biological system, complex data analysis, development of personalized medicine, development of new drugs and therapeutic for real life application



Contents of the hands-on training



Theory lectures: Introduction to animal cell culture and its application, basic requirements for animal cells and different culture techniques, Stem cells in regenerative medicine, Strategies for gene cloning and eukaryotic expression, Heterologous Gene expression in mammalian cell lines and purification of recombinant proteins, Virus isolation in animal cell culture and their titration, Cell viability, proliferation and cytotoxicity assays.

Practical sessions: Sterilization procedures for cell culture work, Preparation of cell culture media and reagents, Handling/ subculture and maintenance of cell lines, Cell counting and cryopreservation of cell lines, Primary culture establishment from Chicken Embryo, Ligation of gene of interest to expression vector and transformation to competent cells, Selection and screening of clones by colony PCR, Extraction of Plasmid and evaluation of its quality and concentration, Transfection of mammalian cell line for protein expression, Detection of expressed protein by GFP/RFP marker and Dot Blot assay, Isolation of virus in animal cell culture, Extraction of Total DNA/RNA from mammalian cells and determination of quality and concentration, Observation of CPE in cell culture and confirmation of virus isolates by PCR/ RT-PCR, Determination of cell proliferation and cytotoxicity by MTT assay, Harvesting and purification of secreted recombinant proteins from the transfected mammalian cells, Characterization of mammalian cell expressed protein by SDS-PAGE & western blotting.

  • Last date of application: 14th June, 2024